Journal: The ISME Journal

Article Title: A 'universal' type II chaperonin PCR detection system for the investigation of Archaea in complex microbial communities

doi: 10.1038/ismej.2011.96

Figure Lengend Snippet: PCR primers used in this study

Article Snippet: 454 pyrosequencing In addition to the clone library, the thermosome PCR product pool generated from the dry diet rumen samples (tcpdry) was sequenced on a 454 GS FLX Titanium instrument (454 Life Sciences, Branford, CT, USA).

Techniques: Sequencing

Journal: The ISME Journal

Article Title: A 'universal' type II chaperonin PCR detection system for the investigation of Archaea in complex microbial communities

doi: 10.1038/ismej.2011.96

Figure Lengend Snippet: Thermosome PCR products from (a) the JH0175/JH0178 primer set, (b) the JH0268/JH0269 primer set and (c) the 7:1 cocktail of JH0175/JH0178:JH0268/JH0269 using 1.0 ng per reaction of genomic DNA from archaeal isolates as templates. The GC content of the species (or genus range, if no complete genome sequence is available for that species) is given in parentheses after the species name. Lanes are (NTC) PCR no template control; (Neg) Escherichia coli DH5α (1) Mc. voltae (28% GC); (2) Mc. vannielii (31% GC); (3) Mc. maripaludis (33% GC); (4) Mt. igneus (38% GC); (5) Ms. hungarei (45% GC); (6) S. solfataricus (36% GC); (7) Sulfolobus sp. (33–36% GC); (8) Tp. acidophilum (46% GC); (9) Tc. gorgonarius (40–54% GC); (10) Tc. pacificus (40–54% GC); (11) Tc. zilligii (40–54% GC); (12) Hb. salinarum (formerly Hb. halobium) (66% GC); (13) Hb. salinarum (formerly Hb. cutirubrum) (66% GC); (14) Hb. salinarum (formerly Hb. salinarium) (66% GC); (15) Hf. volcanii WR341 (66% GC) and (16) Hf. volcanii WR536 (66% GC).

Article Snippet: 454 pyrosequencing In addition to the clone library, the thermosome PCR product pool generated from the dry diet rumen samples (tcpdry) was sequenced on a 454 GS FLX Titanium instrument (454 Life Sciences, Branford, CT, USA).

Techniques: Sequencing, Control